Philipp Christen  .  Rolf Jaussi .  Roger Benoit

Biochemie und Molekularbiologie

Eine Einführung mit 40 Lerneinheiten

11   Regulation der Genexpression


Einführung Website



11.1 Regulation der Transkription bei Prokaryonten: Operon

11.2 Regulation der Transkription bei Eukaryonten: Transkriptionsfaktoren

11.3 Posttranskriptionale Regulation der Genexpression

11.4 Epigenetische Regulation und Vererbung

Weiterführende Literatur


11.1 Regulation der Transkription bei Prokaryonten: Operon

The lac operon ( Animation zum Lac-Operon

Intact lactose operon repressor with gratuitous inducer IPTG ( Struktur des Lac-Repressors

Lac repressor (Protopedia): Struktur des Lac-Repressors

The tryptophan operon ( Trp-Operon Animation

11.2 Regulation der Transkription bei Eukaryonten: Transkriptionsfaktoren

Transcription factors and transcriptional control in eukaryotic cells (Philips and Hoopes 2008, Nature Education 1(1):119): Transkriptionsfaktoren und Transkriptionsregulation in eukaryontischen Zellen

GCN4-pVLT coiled-coil trimer with threonine at the d(12) position (Akey et al 2001, Biochem 40, 6352-6360, RCSB PDB): 3D-Struktur eines trimeren Leu-Zippers: Struktur dreier gleichzeitig an DNA-gebundener DNA-Bindungsdomänen (Custom view: CPK, hydrophobicity)

Helix-turn-helix motif (Protopedia): Struktur eines Helix-Turn-Helix Motivs

DNA microarray technology (National Human Genome Research Institute): Erklärung der "DNA microarray technology"

Transcription complex and enhancers (McGraw Hill): Animation zu Transkriptionskomplex und Enhancern

Structure of the DNA binding domains of IRF2, ATF-2 and Jun bound to DNA (Panne et al. 2004, EMBO J 23, 4384-4393, RCSB PDB)

Master genes control basic body plans ( Animation zur Homöobox von Drosophila (Mastergene)

11.3 Posttranskriptionale Regulation der Genexpression

Video animation: RNA interference (Nature Reviews): Animation zur RNA-Interferenz

Riboswitches: A common RNA regulatory element (Edwards and Batey 2010, Nature Education 3(9):9): Übersicht zu "Riboswitches"

Structure of the S-adenosylhomocysteine riboswitch (Edwards et al. 2010, RNA 16, 2144-2155, RCSB PDB): 3D-Struktur eines Riboswitches, welcher durch Bindung eines Substrats und resultierende Strukturänderungen die Expression eines Gens reguliert

Gene-based therapeutic approaches (Chial 2008, Nature Education 1(1):210): Genbasierte therapeutische Ansätze

11.4 Epigenetische Regulation und Vererbung

Spotlight on Epigenetics (Scitable, Nature Education): Erklärung und Links zum Thema Epigenetik

The histone code (Judd Rice Laboratory): Erklärung des Histon-Codes

Chromatin, histones and modifications (kosigrim, YouTube): Chromatin, Histone und deren Modifikationen

DNA-Methylierung (Wikipedia)

Epigenetik (Wikipedia)

Weiterführende Literatur

Lewis 2013, J Mol Biol - "Allostery and the lac Operon"

Spitz and Furlong 2012, Nat Rev Genet - "Transcription factors: from enhancer binding to developmental control"

Chen 2007, Blood - "Regulation of protein synthesis by the heme-regulated eIF2alpha kinase: relevance to anemias"

Cech 2004, Nature - "RNA finds a simpler way"

Serganov and Nudler 2013, Cell - "A decade of riboswitches"

Aagaard and Rossi 2007, Adv Drug Deliv Rev - "RNAi therapeutics: principles, prospects and challenges"

Hinnebusch et al. 2016, Science - "Translational control by 5'-untranslated regions of eukaryotic mRNAs"

Taft et al. 2009, Nat Genet - "Tiny RNAs associated with transcription start sites in animals"

Tewhey et al. 2016, Cell - "Direct Identification of Hundreds of Expression-Modulating Variants using a Multiplexed Reporter Assay"

Ulirsch et al. 2016, Cell - "Systematic Functional Dissection of Common Genetic Variation Affecting Red Blood Cell Traits"

Mazzio and Soliman 2012, Epigenetics - "Basic concepts of epigenetics: impact of environmental signals on gene expression"

Schreiber 2013, Keystone Symposia - "Epigenetic marks and cancer drugs 2013"

Nacev et al. 2019, Nature - "The expanding landscape of 'oncohistone' mutations in human cancers"

Gallipoli and Huntly 2019, Science - "Histone modifiers are oxygen sensors"

Wilson and Doudna 2013, Annu Rev Biophys - "Molecular mechanisms of RNA interference"

Bensimon et al. 2012, Annu Rev Biochem - "Mass spectrometry-based proteomics and network biology"

Battich et al. 2013, Nat Methods - "Image-based transcriptomics in thousands of single human cells at single-molecule resolution"

Gudjonsson and Krueger 2012, J Invest Dermatol - "A role for epigenetics in psoriasis: methylated Cytosine-Guanine sites differentiate lesional from nonlesional skin and from normal skin"

Sweatt 2016, Science - "GENE EXPRESSION. Chromatin controls behavior"

Weib et al. 2009, BIOspektrum - "Königin oder Arbeiterin? Epigenetik entscheidet"

Revision Mai 2020