Philipp Christen  .  Rolf Jaussi .  Roger Benoit

Biochemie und Molekularbiologie

Eine Einführung mit 40 Lerneinheiten




37   Trennverfahren und allgemeine Analysemethoden


Inhaltsverzeichnis

Einführung Website

Merksätze

Kontrollfragen

37.1 Zentrifugation

37.2 Chromatographie

37.3 Elektrophorese

37.4 Spektroskopie

37.5 Massenspektrometrie

37.6 Isotopenmarkierung, Radionuclide

37.7 Monoklonale Antikörper

37.8 pH-Puffer

Weiterführende Literatur



Links

37.1 Zentrifugation

Cell fractionation (SoKan, YouTube): Differenzielle Zentrifugation von Zellbestandteilen

Centrifugation separations (www.sigmaaldrich.com)

37.2 Chromatographie

Chromatographie (Andreas Meier, YouTube)

Column chromatography (MIT OpenCourseWare, YouTube): Vorlesung zur Säulenchromatographie

Chromatographie von Proteinen (Chemgapedia)

High performance liquid chromatography (This video has been provided via the Royal Society of Chemistry’s YouTube channel): Übersicht zur HPLC

37.3 Elektrophorese

Gelelektrophorese (Chemgapedia)

Elektrophorese (www.med4you.at/)

How to run a 2D-Electrophoresis gel from start to finish (BioRadLifeScience, YouTube): Zweidimensionale Gelelektrophorese

37.4 Spektroskopie

Spektroskopie (Chemgapedia)

Fluoreszenzspektroskopie (Spektrum.de)

Fluoreszenzspektroskopie (Vogel et al., www.biophys.uni-frankfurt.de)

Circulardichroismus und Proteinstruktur (Chemgapedia)

The fluorescent protein color palette (www.olympusconfocal.com): Die Farbpalette der fluoreszierenden Proteine

Fluorescence spectra viewer (www.thermofisher.com)

37.5 Massenspektrometrie

Massenspektrometrie in der Proteindynamik (Chemgapedia)

Massenspektrometrie- und Proteomics-Labor (Universität Bern, Departement Klinische Forschung): Video

Massenspektrometrie (Wikipedia)

Proteomforschung: Einführung (Chemgapedia)

37.6 Isotopenmarkierung, Radionuclide

Karlsruher Nuklidkarte (Wikipedia)

Isotopenmarkierung (Chemgapedia)

Radiolabeling and isotopic markers (Matthew Schlecht, PhD; www.radiolabeling.org)

Isotopic labelling (V.A. Higman; www.protein-nmr.org.uk): Isotopenmarkierungen für Protein-NMR

37.7 Monoklonale Antikörper

Enzyme-linked immunosorbent assay- ELISA (C. Engleberg, openmichigan, YouTube): Einfache Animation

Enzyme linked immunosorbent assay (Chemgapedia): ELISA (und Radioimmunoassay RIA)

Enzyme-linked immunosorbent assay (Wikipedia): ELISA

37.8 pH-Puffer

Pufferlösungen (Chemgapedia)

pH-Stat Theory and practice (IGZ Instruments AG, www.igz.ch)

Buffers (MIT OpenCourseWare, YouTube)

Buffer Reference Center (www.sigmaaldrich.com): Gebräuchliche Puffer



Weiterführende Literatur

Satori et al. 2012, Anal Chim Acta - "Review on recent advances in the analysis of isolated organelles"

de Araujo et al. 2008, Methods Mol Biol - "Isolation of endocitic organelles by density gradient centrifugation"

Maeda et al. 2014, Nat Protoc - "A generic protocol for the purification and characterization of water-soluble complexes of affinity-tagged proteins and lipids"

Minton 1990, Anal Biochem - "Quantitative characterization of reversible molecular associations via analytical centrifugation"

Labrou 2014, Methods Mol Biol - "Protein purification: an overview"

Mitulovic and Mechtler 2006, Brief Funct Genomic Proteomic - "HPLC techniques for proteomics analysis--a short overview of latest developments"

Hirabayashi et al 2015, Molecules - "The Lectin Frontier Database (LfDB), and Data Generation Based on Frontal Affinity Chromatography"

Stellwagen 2009, Electrophoresis - "Electrophoresis of DNA in agarose gels, polyacrylamide gels and in free solution"

Stead and McDowall 2007, Nat Protoc - "Two-dimensional gel electrophoresis for identifying proteins that bind DNA or RNA"

Rogowska-Wrzesinska et al. 2013, J Proteomics - "2D gels still have a niche in proteomics"

Walter et al. 2008, Nat Methods - "Do-it-yourself guide: how to use the modern single-molecule toolkit"

Pennisi 2016, Science - "‘Cell painting’ highlights responses to drugs and toxins"

Andresen et al. 2016, BIOspektrum - "Reversibel schaltbare fluoreszierende Proteine für die Superauflösung"

Neira 2013, Subcell Biochem - "Fluorescence, circular dichroism and mass spectrometry as tools to study virus structure"

Peterman et al. 2004, Annu Rev Phys Chem - "Single-molecule fluorescence spectroscopy and microscopy of biomolecular motors"

Branigan 2013, Nat Protoc - "Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding"

Whitmore and Wallace 2008, Biopolymers - "Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases"

Shaner et al 2005, Nat Methods - "A guide to choosing fluorescent proteins"

Yates et al. 2009, Annu Rev Biomed Eng - "Proteomics by mass spectrometry: approaches, advances, and applications"

Bantscheff et al. 2012, Anal Bioanal Chem - "Quantitative mass spectrometry in proteomics: critical review update from 2007 to the present"

Matthews 2007, J Nutr - "An overview of phenylalanine and tyrosine kinetics in humans"

Ecker and Liebisch 2014, Prog Lipid Res - "Application of stable isotopes to investigate the metabolism of fatty acids, glycerophospholipid and sphingolipid species"

Boersema et al 2009, Nat Protoc - "Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics"

Sugiura et al 2014, Molecules - "Radiolabeling strategies for tumor-targeting proteinaceous drugs"

Marks and Nolan 2006, Nat Methods - "Chemical labeling strategies for cell biology"

Liu 2014, Ann Med Surg (Lond) - "The history of monoclonal antibody development - Progress, remaining challenges and future innovations"

Razinkov et al. 2015, J Biomol Screen - "Accelerated Formulation Development of Monoclonal Antibodies (mAbs) and mAb-Based Modalities: Review of Methods and Tools"

Mach 2012, Cancer Immun - "Introduction to monoclonal antibodies"


Revision November 2018