Philipp Christen  .  Rolf Jaussi .  Roger Benoit

Biochemie und Molekularbiologie

Eine Einführung mit 40 Lerneinheiten




9   Transkription: Biosynthese der RNA


Inhaltsverzeichnis

Einführung Website

Merksätze

Kontrollfragen

9.1 Initiation

9.2 Elongation und Termination

9.3 Modifikationen des primären Transkriptionsprodukts

9.4 Spleißen (Splicing)

9.5 Synthese der tRNA und rRNA

Weiterführende Literatur



Links

9.1 Initiation

Transcription initiation by sigma54 (cchem.berkeley.edu, Prof. Wemmer): Übersicht zur Funktion verschiedener Sigma-Faktoren

TATA-binding protein (David S. Goodsell, RCSB PDB): Beschreibung und Struktur des TATA-Faktors

Hemmstoffe der Transkription

Transcription complex and enhancers (McGraw Hill): Animation zur Initiation der Transkription

Structure of an initially transcribing RNA polymerase II-TFIIB complex (Sainsbury et al. 2013, Nature 493, 437; RCSB PDB): Struktur eines RNA Polymerase II - TFIIB Komplexes zu Beginn der Transkription

Enhanceosome (David S. Goodsell, RCSB PDB): Struktur von Proteinen, die als Enhancer wirken

Oct and sox transcription factors (David S. Goodsell, RCSB PDB): Struktur von Transkriptonsfaktoren

9.2 Elongation und Termination

RNA transcription by RNA polymerase: procaryotes vs eukaryotes (Scitable - Nature): Vergleich der prokaryontischen und eukaryontischen Transkription

RNA polymerase (David S. Goodsell, RCSB PDB): Beschreibung und Struktur der RNA-Polymerase

DNA transcription - basic detail (Howard Hughes Medical Institute): Animation zur Transkription

9.3 Modifikationen des primären Transkriptionsprodukts

mRNA processing (Virtual cell animation collection): Animation zur Prozessierung der mRNA

Poly(A) polymerase (David S. Goodsell, RCSB PDB): Übersicht und Struktur der Poly(A)-Polymerase

9.4 Spleißen (Splicing)

Evolution von Introns und Ribozymen

RNA splicing (dnalc.org): Einfache Animation des Prinzips von mRNA-Spleißen

3D Animation of RNA splicing (dnalc.org): 3D-Animation des mRNA-Spleißen

9.5 Synthese der tRNA und rRNA

Transfer RNA (scindiaprem, YouTube): Architektur der tRNA


Weiterführende Literatur

Schilbach et al. 2017, Nature - "Structures of transcription pre-initiation complex with TFIIH and Mediator"

Taatjes et al. 2004, Nat Rev Mol Cell Biol - "Regulatory diversity among metazoan co-activator complexes"

Riethoven 2010, Methods Mol Biol - "Regulatory regions in DNA: promoters, enhancers, silencers, and insulators"

Cramer 2019, Nature - "Organization and regulation of gene transcription"

Licatalosi and Darnell 2010, Nat Rev Genet - "RNA processing and its regulation: global insights into biological networks"

Fouqueau and Werner 2017, Science - "The architecture of transcription elongation"

Pennisi 2012, Science -"Genomics. ENCODE project writes eulogy for junk DNA"

Han et al. 2016, Biomed Res Int - "Long Noncoding RNA Identification: Comparing Machine Learning Based Tools for Long Noncoding Transcripts Discrimination"

Weinberg and Gross 2017, Nature - "Molecular biology: Messenger RNAs marked for longer life"

Riddihough 2016, Science - "Signals in RNA"

Harcourt et al. 2017, Nature - "Chemical and structural effects of base modifications in messenger RNA"

Yan et al. 2017, Science - "Structure of a yeast step II catalytically activated spliceosome"

Kosmyna and Query 2016, Nature - "Structural biology: Catalytic spliceosome captured"

Traunmüller et al. 2016, Science - "Control of neuronal synapse specification by a highly dedicated alternative splicing program"

Cate 2016, Science - "STRUCTURE. A Big Bang in spliceosome structural biology"

Sainsbury et al. 2013, Nature - "Structure and function of the initially transcribing RNA polymerase II-TFIIB complex"

Ren et al. 2013, Proteins -"Structure of the RNA-directed RNA polymerase from the cystovirus Phi 12"


Revision August 2022