Philipp Christen  .  Rolf Jaussi .  Roger Benoit

Biochemie und Molekularbiologie

Eine Einführung mit 40 Lerneinheiten




4   Enzyme


Inhaltsverzeichnis

Einführung Website

Merksätze

Kontrollfragen

4.1 Allgemeine Eigenschaften der Enzyme

4.2 Katalyse und Aktivierungsenergie

4.3 Enzymkinetik

4.4 Struktur der aktiven Stelle und Wirkungsmechanismus von Enzymen

4.5 Beispiele von Enzymmechanismen

4.6 Regulation der Enzymaktivität

Weiterführende Literatur

Links

4.1 Allgemeine Eigenschaften der Enzyme

Enzymes (David S. Goodsell, RCSB PDB): Struktur und Funktion verschiedener Enzyme (Unter "Molecules of Life" - "Enzymes")

EMBL-EBI Database

BRENDA Enzyme Database

Expasy enzyme nomenclature database

Neuronal signaling and the sodium-potassium pump (RCSBProteinDataBank, YouTube): Übersicht zum Mechanismus der Natrium-Kalium Pumpe

The sodium-potassium pump (getbotysmart.com): Animation zum Mechanismus der Natrium-Kalium Pumpe

4.2 Katalyse und Aktivierungsenergie

Biochemistry enzyme mechanism proteases tutorial (P.M. Bingham, Streaming Tutors, YouTube): Vorlesung zur Katalyse

Introduction to chemical biology 128 - lecture 14 - glycobiology (University of California Irvine, open course ware, YouTube): Vorlesung zur Glykobiologie

4.3 Enzymkinetik

How enzymes work (RCSBProteinDataBank, YouTube)

Bestimmung von Km und Vmax (Bipweb)

4.4 Struktur der aktiven Stelle und Wirkungsmechanismus von Enzymen

DNA Ligase (David S. Goodsell, RCSB PDB)

DNA Polymerase (David S. Goodsell, RCSB PDB)

RNA Polymerase (David S. Goodsell, RCSB PDB)

Restriction enzymes (David S. Goodsell, RCSB PDB)

Reverse transcriptase (from HIV) (David S. Goodsell, RCSB PDB)

Pyruvatkinase (Biplex)

Aldolase (Biplex)

4.5 Beispiele von Enzymmechanismen

Serine protease mechanism (biology.kenyon.edu, Anna Paige): Mechanismus der Serin-Proteasen

Bovine chymotrypsin complexed to appi (Scheidig and Kossiakoff, RCSB PDB): Serin-Proteasen Struktur

4.6 Regulation der Enzymaktivität

Enzyme function and inhibition (YouTube)

Weiterführende Literatur

Du et al. 2025, Science - "Conformational ensembles reveal the origins of serine protease catalysis"

Lemke et al. 2025, Nature - "The role of metabolism in shaping enzyme structures over 400 million years"

Fas et al. 2025, Curr Opin Struct Biol - "Global dynamics behind enzyme catalysis, evolution, and design"

Braun et al. 2026, Nature - "Computational enzyme design by catalytic motif scaffolding"

Dreifke and Fröba 2017, BIOspektrum - "Immobilisierung von Enzymen: Spielerei oder biotechnologischer Fortschritt?"

Kirk et al. 2002, Curr Opin Biotechnol - "Industrial enzyme applications"

Nashine et al. 2010, Curr Opin Chem Biol - "Coupled motions in enzyme catalysis"

Glowacki et al. 2012, Nat Chem - "Taking Ockham's razor to enzyme dynamics and catalysis"

Villali and Kern 2010, Curr Opin Chem Biol - "Choreographing an enyzme's dance"

Harris and Keshwani 2009, Methods Enzymol - "Measurement of enzyme activity"

Eliot and Kirsch 2004, Annu Rev Biochem - "Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations"

Mehta and Christen 2000, Adv Enzymol Relat Areas Mol Biol - "The molecular evolution of pyridoxal-5'-phosphate-dependent enzymes"

Almonte and Sweatt 2011, Brain Res - "Serine proteases, serine protease inhibitors, and protease-activated receptors: roles in synaptic function and behavior"

Hedstrom 2002, Chem Rev - "Serine protease mechanism and specificity"

Nguyen et al. 2017, Science - "Evolutionary drivers of thermoadaptation in enzyme catalysis"

Saleh and Kalodimos 2017, Science - "Enzymes at work are enzymes in motion"

Jencks 1969 - "Catalysis in chemistry and enzymology" (Buch, Enzymologie-Klassiker): Link zu Google books

Juretschke and Deuerling 2012, Springer Verlag BIOspektrum - "Ribosom-assoziierte Chaperone kontrollieren die Proteinsynthese"

Motlagh et al. 2014, Nature - "The ensemble nature of allostery"


Revision Januar 2026