Philipp Christen  .  Rolf Jaussi .  Roger Benoit

Biochemie und Molekularbiologie

Eine Einführung mit 40 Lerneinheiten




8   Replikation, Reparatur und Rekombination der DNA


Inhaltsverzeichnis

Einführung Website

Merksätze

Kontrollfragen

8.1 DNA-Replikation bei Prokaryonten

8.2 DNA-Replikation bei Eukaryonten

8.3 DNA-Schäden und Reparatursysteme

8.4 Genetische Rekombination

Weiterführende Literatur

Links

8.1 DNA-Replikation bei Prokaryonten

DNA replication (Howard Hughes Medical Institute): Animation zur Replikation von DNA

DNA polymerase (David S. Goodsell, RCSB PDB): Struktur und Einführung zu DNA-Polymerase

Topoisomerases (David S. Goodsell, RCSB PDB): Übersicht zur Struktur und Funktion der Topoisomerasen

8.2 DNA-Replikation bei Eukaryonten

Structure of human DNA polymerase beta complexed with DNA (Pelletier and Sawaya 1996, Biochemistry 35, 12778-12787, RCSB PDB): Struktur der menschlichen DNA-Polymerase Beta im Komplex mit DNA

Telomerase database (https://telomerase.us/): Telomerase-Datenbank - Struktur, Funktion und Evolution der Telomerase-Ribonucleoproteine

Genetischer Code (Wikipedia)

8.3 DNA-Schäden und Reparatursysteme

What kinds of gene variants are possible? (https://medlineplus.gov/): Kurzübersicht zu möglichen Mutationen

Endogene und exogene Faktoren, die zu Mutationen führen können; Ames-Test auf mutagene Wirkung

Thymine dimers (David S. Goodsell, RCSB PDB: Struktur und Erklärung der Thymindimere

DNA replication and causes of mutation (Pray 2008, Nature Education 1(1)214): Übersicht zu DNA-Korrekturlesemechanismen

DNA damage & repair: Mechanisms for maintaining DNA integrity (Clancy 2008, Nature Education 1(1)103): Mechanismen der Reparatur von DNA Schäden

Xeroderma pigmentosum

8.4 Genetische Rekombination

Resolving Holliday Structures 1 (UF Teaching Center, YouTube): Animation der homologen Rekombination

DNA Holliday junction (YouTube): Animation zur Teilung der Holliday-Struktur

Transposase (David S. Goodsell, RCSB PDB): Struktur und Funktion der Transposase

How alu jumps (dnalc.org): Animation zu einem Mechanismus der Transposition

Transposase (B. DesPain, M. Campbell, bio.davidson.edu): Geschichte der Entdeckung der Transposons mit Struktur

Weiterführende Literatur

Berkemeier et al. 2025, Nat Commun - "DNA replication timing reveals genome-wide features of transcription and fragility"

Carrington et al. 2025, Genome Biol - "Most human DNA replication initiation is dispersed throughout the genome with only a minority within previously identified initiation zones"

Ceccaldi and Cejka 2025, Nat Rev Mol Cell Biol - "Mechanisms and regulation of DNA end resection in the maintenance of genome stability"

Chiolo et al. 2025, Nat Rev Mol Cell Biol - "Nuclear and genome dynamics underlying DNA double-strand break repair"

Van Sluis et al. 2025, Nat Rev Mol Cell Biol - "Mechanisms of transcription-coupled repair and DNA damage surveillance in health and disease"

Hübscher 2009, Methods Mol Biol - "DNA replication fork proteins"

Hamers 2016, Science - "EVOLUTION. Why do cells' power plants hang on to their own genomes?"

Wallace 2016, Nature - "Genetics: Mitochondrial DNA in evolution and disease"

Shoubridge 2016, Nature - "Biomedicine: Replacing the cell's power plants"

Marnett and Plastaras 2001, Trends Genet - "Endogenous DNA damage and mutation"

De Bont and Larebeke 2004, Mutagenesis - "Endogenous DNA damage in humans: a review of quantitative data"

Hoeijmakers 2001, Nature - "Genome maintenance mechanisms for preventing cancer"

Wells and Watt 2018, Nature - "Diverse mechanisms for endogenous regeneration and repair in mammalian organs"

Xu et al. 2010, Science - "Partitioning of histone H3-H4 tetramers during DNA replication-dependent chromatin assembly"

Kumar and Subramanian 2002, Proc Natl Acad Sci USA - "Mutation rates in mammalian genomes"

Kim and Wilson 2012, Curr Mol Pharmacol - "Overview of base excision repair biochemistry"

McKinnon and Caldecott 2007, Annu Rev Genomics Hum Genet - "DNA strand break repair and human genetic disease"

Price and D'Andrea 2013, Cell - "Chromatin remodeling at DNA double-strand breaks"

Friedberg 2003, Nature - "DNA damage and repair"

Friedberg 2008, Cell Res - "A brief history of the DNA repair field"

Kunkel and Erie 2005, Annu Rev Biochem - "DNA mismatch repair"

Li and Heyer 2008, Cell Res - "Homologous recombination in DNA repair and DNA damage tolerance"

Peña-Diaz and Jiricny 2012 - "Mammalian mismatch repair: error-free or error-prone?"

Kad and Van Houten 2016, Nature - "DNA repair: Clamping down on copy errors"

Scott and Devine 2017, Viruses - "The Role of Somatic L1 Retrotransposition in Human Cancers"

Huang and Zhou 2021, Nature - "DNA damage repair: historical perspectives, mechanistic pathways and clinical translation for targeted cancer therapy"


Revision Januar 2026